Detailed view for Tb927.8.3350

Basic information

TDR Targets ID: 13949
Trypanosoma brucei, DNA polymerase theta (helicase domain only), putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.407 | Length (AA): 1057 | MW (Da): 115094 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00270   DEAD/DEAH box helicase
PF00271   Helicase conserved C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0006139   nucleobase, nucleoside, nucleotide and nucleic acid metabolic process  
GO:0005575   cellular_component  
GO:0008026   ATP-dependent helicase activity  
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0004386   helicase activity  
GO:0003676   nucleic acid binding  
GO:0000166   nucleotide binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127591)

Species Accession Gene Product
Arabidopsis thaliana AT4G32700   MUS308 and mammalian DNA polymerase-like protein
Brugia malayi Bm1_05460   Type III restriction enzyme, res subunit family protein
Brugia malayi Bm1_47470   Helicase conserved C-terminal domain containing protein
Caenorhabditis elegans CELE_W03A3.2   Protein POLQ-1
Caenorhabditis elegans CELE_Y55B1AL.3   Protein HELQ-1, isoform B
Cryptosporidium hominis Chro.20448   DNA helicase HEL308
Cryptosporidium parvum cgd2_4190   DEXDc+HELICc, mus308/POLQ like SFII DNA helicase, no polymerase domain
Dictyostelium discoideum DDB_G0279107   hypothetical protein
Drosophila melanogaster Dmel_CG7972   mutagen-sensitive 301
Drosophila melanogaster Dmel_CG6019   mutagen-sensitive 308
Echinococcus granulosus EgrG_000705300   DNA polymerase theta
Echinococcus granulosus EgrG_000593900   helicase polq
Entamoeba histolytica EHI_007040   hypothetical protein
Echinococcus multilocularis EmuJ_000705300   DNA polymerase theta
Echinococcus multilocularis EmuJ_000593900   helicase polq
Giardia lamblia GL50803_11384   DNA helicase
Homo sapiens ENSG00000163312   helicase, POLQ-like
Homo sapiens ENSG00000051341   polymerase (DNA directed), theta
Leishmania braziliensis LbrM.23.1500   DNA polymerase theta (helicase domain only), putative
Leishmania donovani LdBPK_231640.1   DNA polymerase theta (helicase domain only), putative
Leishmania infantum LinJ.23.1640   DNA polymerase theta (helicase domain only), putative
Leishmania major LmjF.23.1380   DNA polymerase theta (helicase domain only), putative
Leishmania mexicana LmxM.23.1380   DNA polymerase theta (helicase domain only), putative
Loa Loa (eye worm) LOAG_08332   hypothetical protein
Loa Loa (eye worm) LOAG_12396   hypothetical protein
Loa Loa (eye worm) LOAG_04395   hypothetical protein
Loa Loa (eye worm) LOAG_03612   hypothetical protein
Mus musculus ENSMUSG00000035266   helicase, POLQ-like
Mus musculus ENSMUSG00000034206   polymerase (DNA directed), theta
Onchocerca volvulus OVOC13445  
Schistosoma japonicum Sjp_0113090   ko:K02349 DNA polymerase theta subunit, putative
Schistosoma japonicum Sjp_0096710   ko:K01509 adenosinetriphosphatase [EC3.6.1.3], putative
Schistosoma japonicum Sjp_0095310   ko:K02349 DNA polymerase theta subunit, putative
Schistosoma japonicum Sjp_0057080   ATP-dependent DNA helicase HEL308, putative
Schistosoma mansoni Smp_155500   DNA polymerase theta
Schistosoma mansoni Smp_019760   DNA polymerase theta
Schmidtea mediterranea mk4.002242.00   Helicase POLQ-like
Schmidtea mediterranea mk4.002878.02   Helicase POLQ-like
Schmidtea mediterranea mk4.002067.00   Helicase POLQ-like
Schmidtea mediterranea mk4.000147.03   Helicase POLQ-like
Trypanosoma brucei gambiense Tbg972.8.3110   DNA polymerase theta (helicase domain only), putative
Trypanosoma brucei Tb927.8.3350   DNA polymerase theta (helicase domain only), putative
Trypanosoma brucei Tb11.v5.0792   DNA polymerase theta (helicase domain only), putative
Trypanosoma congolense TcIL3000_8_3410   DNA polymerase theta (helicase domain only), putative
Trypanosoma cruzi TcCLB.509769.70   DNA polymerase theta (helicase domain only), putative
Trichomonas vaginalis TVAG_278590   conserved hypothetical protein
Trichomonas vaginalis TVAG_268100   helicase, putative
Trichomonas vaginalis TVAG_246950   helicase, putative
Trichomonas vaginalis TVAG_042000   DNA polymerase theta, putative
Trichomonas vaginalis TVAG_016690   conserved hypothetical protein
Trichomonas vaginalis TVAG_016720   conserved hypothetical protein
Trichomonas vaginalis TVAG_475620   activating signal cointegrator 1 complex subunit 3, helc1, putative
Trichomonas vaginalis TVAG_274650   activating signal cointegrator 1 complex subunit 3, helc1, putative

Essentiality

Tb927.8.3350 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.3350 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.3350 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.3350 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.3350 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.8.3350 (Trypanosoma brucei), DNA polymerase theta (helicase domain only), putative
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